Escherichia coli Nissle 1917 (EcN) is a non-pathogenic E. coli strain that confers health benefits without known harmful effects on various hosts and is marketed under the trade name Mutaflor (Sonnenborn and Schulze2009). FirstisolatedbyDr.AlfredNissleduringWorldWarI, EcN has proven to be effective at competing with other Escherichia coli Nissle 1917 (EcN) bears a defect in its LPS biosynthesis leading to truncated variable oligosaccharide-antigen chains and a semi-rough phenotype. It is effectively inactivated by complement factors due to resolved serum resistance and is, therefore, safe as a probiotic strain, i.e. for the treatment of inflammatory Jun 5, 2023 · Escherichia coli Nissle 1917 (EcN) is a probiotic microbe that has the potential to be developed as a promising chassis for synthetic biology applications. However, the molecular tools and techniques for utilizing EcN have not been fully explored. To address this opportunity, we systematically expanded the EcN-based toolbox, enabling EcN as a powerful platform for more applications. First, two Oct 6, 2020 · We have recently shown that E. coli Nissle 1917 (EcN) is a very suitable host for the production of heme proteins . In this study, we went a step further and constructed a T7 promoter compatible strain to overcome the limitation of recombinant protein production in EcN to expression vectors lacking the T7 promoter. Oct 1, 2019 · Extracellular membrane vesicles (MVs) released by gut microbiota are key players in the communication with the host. The aim of this study was to evaluate the immunomodulatory properties of MVs from the probiotic E. coli Nissle 1917 (EcN) in terms of DC-derived adaptive immune responses and to compare the effects with those elicited by commensal E. coli. Apr 8, 2020 · Guo, S. et al. Escherichia coli Nissle 1917 protects intestinal barrier function by inhibiting NF-κB-mediated activation of the MLCK-P-MLC signaling pathway. Mediators Inflamm . 2019 , 5796491 May 29, 2018 · Shiga toxin (Stx) producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC) are the major cause of foodborne illness in humans. In vitro studies showed the probiotic Escherichia coli strain Nissle 1917 (EcN) to efficiently inhibit the production of Stx. Life threatening EHEC strains as for example the serotype O104:H4, responsible for the great outbreak in 2011 in Germany, evolutionary May 1, 2020 · In 1917, Escherichia coli Nissle was isolated from the feces of a German soldier who claimed to be covered from infectious diarrheal sicknesses. From that point forward, EcN has been widely studied, and a specific trend of fitness factors as the undeniable absence of harmfulness factors have risen. Jan 1, 2021 · Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium originally isolated from a particularly healthy soldier from World War I by the physician Alfred Nissle. 1 Since then, this bacterium has found significant use as a probiotic therapy, outcompeting pathogens in the gut 2 and thus protecting the host from infection. Mar 22, 2021 · The probiotic Escherichia coli strain Nissle 1917 (DSM 6601, Mutaflor), generally considered as beneficial and safe, has been used for a century to treat various intestinal diseases. However, Nissle 1917 hosts in its genome the pks pathogenicity island that codes for the biosynthesis of the genotoxin colibactin. Colibactin is a potent DNA alkylator, suspected to play a role in colorectal k3rNG.